Transformation Fava Beans by Agrobacterium using Chitinase, Glucanase and CryIA (b) genes
نویسندگان
چکیده
منابع مشابه
Transformation of Potato (Solanum tuberosumcv.Savalan) by Chitinase and β-1,3-Glucanase Genes of Myco-Parasitic Fungi Towards Improving Resistance to Rhizoctonia solani AG-3
Potato (Solanum tuberosum L.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including Rhizoctonia solani (AG-3), the causal agent of stem and root rot diseases. Chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. In the present study, plasmid p...
متن کاملAgrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer.
Over 2,600 transgenic rice plants in nine strains were regenerated from >500 independently selected hygromycin-resistant calli after Agrobacterium-mediated transformation. The plants were transformed with fully modified (plant codon optimized) versions of two synthetic cryIA(b) and cryIA(c) coding sequences from Bacillus thuringiensis as well as the hph and gus genes, coding for hygromycin phos...
متن کاملtransformation of potato (solanum tuberosumcv.savalan) by chitinase and î²-1,3-glucanase genes of myco-parasitic fungi towards improving resistance to rhizoctonia solani ag-3
potato (solanum tuberosum l.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including rhizoctonia solani (ag-3), the causal agent of stem and root rot diseases. chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. in the present study, plasmid p...
متن کاملGenetic transformation of Tomato with three pathogenesis-related protein genes for increased resistance to Fusarium oxysporum f.sp. lycopersici
Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre-culture me...
متن کاملDifferential Expression of Chitinase and ß-l,3-Glucanase Genes in Various Tissues of Potato Plants
We have analyzed the tissue-specific expression o f chitinase, ß-l,3-glucanase and, for com parison, phenylalanine am m onia-lyase genes in healthy, unstressed po ta to (Solanum tubero sum) plants by using R N A -R N A hybridization and im m unostaining techniques to localize the respective m R N A s and proteins in situ. P reparations o f leaf, flower, shoot tip, node and axillary bud revea...
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ژورنال
عنوان ژورنال: International Journal on Advanced Science, Engineering and Information Technology
سال: 2014
ISSN: 2460-6952,2088-5334
DOI: 10.18517/ijaseit.4.2.366